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He was born in Van Buren, Arkansas on August 4, His life was his family, church, and work. He was a respected and beloved educator serving Fort Smith public schools for 34 years. He directed bands in Dardanelle, Marianna, Smackover and Fort Smith, AR influencing hundreds of students as teacher, mentor and friend. His award-winning bands at Northside High School, Fort Smith, set the standard of music excellence and were highly regarded, performing for two presidents during his tenure.
A decline in chloroplast DNA cpDNA during leaf maturity has been reported previously for eight plant species, including Arabidopsis thaliana. Recent studies, however, concluded that the amount of cpDNA during leaf development in Arabidopsis remained constant. To evaluate alternative hypotheses for these two contradictory observations, we examined cpDNA in Arabidopsis shoot tissues at different times during development using several methods: staining leaf sections as well as individual isolated chloroplasts with 4',6-diamidinophenylindole DAPI , real-time quantitative PCR with DNA prepared from total tissue as well as from isolated chloroplasts, fluorescence microscopy of ethidium-stained DNA molecules prepared in gel from isolated plastids, and blot-hybridization of restriction-digested total tissue DNA. We observed a developmental decline of about two- to three-fold in mean DNA per chloroplast and two- to five-fold in the fraction of cellular DNA represented by chloroplast DNA.
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